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Temperature modulate disease susceptibility of the Pacific oyster Crassostrea gigas and virulence of the Ostreid herpesvirus type 1 ArchiMer
Delisle, Lizenn; Petton, Bruno; Burguin, Jean Francois; Morga, Benjamin; Corporeau, Charlotte; Pernet, Fabrice.
Temperature triggers marine diseases by changing host susceptibility and pathogen virulence. Oyster mortalities associated with the Ostreid herpesvirus type 1 (OsHV-1) have occurred seasonally in Europe when the seawater temperature range reaches 16–24 °C. Here we assess how temperature modulates oyster susceptibility to OsHV-1 and pathogen virulence. Oysters were injected with OsHV-1 suspension incubated at 21 °C, 26 °C and 29 °C and were placed in cohabitation with healthy oysters (recipients) at these three temperatures according to a fractional factorial design. Survival was followed for 14 d and recipients were sampled for OsHV-1 DNA quantification and viral gene expression. The oysters were all subsequently placed at 21 °C to evaluate the potential...
Tipo: Text Palavras-chave: Bivalve; Health; Marine disease; Mortality risk; Temperature; Virulence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00442/55337/56847.pdf
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Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids ArchiMer
Dufy, Christian; Diter, Alain.
An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean...
Tipo: Text Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf
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Etat des connaissances sur la pathologie chez les mollusques bivalves ArchiMer
Fouche, Dominique.
A short description of the disease causing organisms affecting bivalves is presented in this document, including the diagnosis, the oustanding research and bivalve husbandry. Mollusc populations may be safe from disease epizootics if an early prevention of disease is coupled with a strict regulatory system on transfer (according to what happens in agriculture). The species to culture may be thoroughly selected and risky populations moved. Imported stocks are strictly controlled. The researchcs's target is to improve the diagnosis tools and try to find resistant mollusc strains. Epidemiological surveys are also necessary to control diseases.
Tipo: Text Palavras-chave: Transfer; Husbandry; Prophylatics; Bivalve; Protozoan; Virus; Bacteria; Metazoan; Transfert; Zootechnie; Prophylaxie; Bivalve; Protozoaire; Virus; Bactérie; Métazoaire.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/rapport-1923.pdf
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Bacterial problems associated with scallop Pecten maximus larval culture ArchiMer
Nicolas, Jean-louis; Corre, S; Gauthier, Gilles; Robert, Rene; Ansquer, Dominique.
Scallop Pecten maximus larvae have been cultured at the Argenton and Tinduff (Brittany, France) hatcheries with antibiotic treatment (chloramphenicol at 8 ppm) for 15 yr. Without treatment, outbreak of disease has normally occurred between Day 12 and Day 19 or sometimes earlier. A bacteriological study of larvae reared with and without antibiotic was performed over a 4 yr period. Among the collected strains, 2 clusters (C and F) of vibrios were present at high densities only in larvae cultured without treatment. One cluster (C) was routinely isolated over the 4 yr of study, while the other (F) was collected only in the third year. Their virulence with respect to scallop larvae and their lack of infectivity with respect to oyster larvae were demonstrated in...
Tipo: Text Palavras-chave: Larvae; Bivalve; Vibrio; Scallop.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00090/20077/17716.pdf
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The future is now: marine aquaculture in the anthropocene ArchiMer
Pernet, Fabrice; Browman, Howard I.
Aquaculture now produces more seafood than wild capture fisheries and this production is expected to at least double by 2050. Representing almost half of global production, marine aquaculture will contribute to sustainably feeding the growing humanity. However, climate change will undoubtedly challenge the future growth of marine aquaculture. Temperature and sea-level rise, shifts in precipitation, freshening from glacier melt, changing ocean productivity, and circulation patterns, increasing occurrence of extreme climatic events, eutrophication, and ocean acidification are all stressors that will influence marine aquaculture. The objective of this themed article set was to bring together contributions on the broad theme of the potential impacts,...
Tipo: Text Palavras-chave: Adaptation; Bivalve; Blue economy; Carbon; Climate change; Marine diseases; Mitigation; Ocean acidification; Plasticity; Salmon; Seaweed; Sustainability.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00682/79406/81951.pdf
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Infectious diseases in oyster aquaculture require a new integrated approach ArchiMer
Pernet, Fabrice; Lupo, Coralie; Bacher, Cedric; Whittington, Richard J..
Emerging diseases pose a recurrent threat to bivalve aquaculture. Recently, massive mortality events in the Pacific oyster Crassostrea gigas associated with the detection of a microvariant of the ostreid herpesvirus 1 (OsHV-1 µVar) have been reported in Europe, Australia and New Zealand. Although the spread of disease is often viewed as a governance failure, we suggest that the development of protective measures for bivalve farming is presently held back by the lack of key scientific knowledge. In this paper, we explore the case for an integrated approach to study the management of bivalve disease, using OsHV-1 as a case study. Reconsidering the key issues by incorporating multidisciplinary science could provide a holistic under-standing of OsHV-1 and...
Tipo: Text Palavras-chave: Bivalve; Disease management; Epidemiology; Multidisciplinary; Risk.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00314/42537/41912.pdf
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Aspects économiques et techniques de la perliculture en Polynésie Française. Contribution à l'étude de l'alimentation de l'huître perlière Pinctada margaritifera ArchiMer
Hautefeuille, Fanny.
Cultured black pearls are now the tirst export value of French Polynesia. This fast growing activity encourage the retum of the islanders to their native islands and the revival of the economy of the archipelagoes. However, the importance of the development induced sorne technical and economical problems. New reglementations are needed to organize the market and stabilize the black pearl price, the quotation of which is falling since 1990. After the important mortalities which occured in 1985-1 986, the mother of pearl general investigation program was set up. Il aims at the study of the biology and the ecology of the mother of pearl Pinclada margaritifera. The study of the nutrition of this oyster is part of this program and will contribute to the...
Tipo: Text Palavras-chave: Perliculture; Polynésie Française; Problèmes; Bivalve; Huître perlière; Pinctada margaritifera; Bilan énergétique; Contenu stomacal; Assimilation; Conover; Perliculture; French Polynesia; Problems; Bivalvia; Pearl oyster; Pinctada margaritifera; Energetic balance; Stomacal contents; Assimilation; Conover.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00424/53540/54397.pdf
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Remodeling of membrane lipids in gills of adult hard clam Mercenaria mercenaria during declining temperature ArchiMer
Parent, G; Pernet, Fabrice; Tremblay, R; Sevigny, J; Ouellette, M.
In a previous study, a major remodeling of lipids, consistent with the theory of homeoviscous adaptation, was observed in the juvenile hard clam Mercenaria mercenaria during a temperature reduction from 24 to -1 degrees C. In addition, the lipid remodeling varied between genetically distinct lines of hard clams. The present study examined whether adult hard clams originating from different locations and a selectively bred variety show differences related to their genetic characteristics in the remodeling of lipids that normally occur during decreasing temperatures. Wild hard clams from 4 locations in Atlantic Canada and the selectively bred M mercenaria var. notata were held at an aquaculture growout site located at the northern distribution limit of the...
Tipo: Text Palavras-chave: Sterol; Phospholipid; Membrane lipid; Intraspecific variation; Food; Fatty acid; Bivalve; Allozymes; Acclimatization.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4743.pdf
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Etude des interactions hôte/virus chez l’huître creuse Crassostrea gigas et son virus Ostreid herpesvirus 1 ArchiMer
Segarra, Amelie.
Ostreid herpesvirus type 1 (OsHV-1) can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas, in France and all around the world. Differences in terms of mortality among infected animals were reported suggesting a genetic basis of the susceptibility to the OsHV-1 infection. In this context, the main objective of this thesis was to understand the interactions between Pacific oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Results showed that the virus is able to replicate in the host regardless of its stage of development or its susceptibility. However, multiplication kinetics is faster in the most susceptible individuals compared to less susceptible ones. After an active replication phase, it...
Tipo: Text Palavras-chave: Bivalves; Huître creuse; Crassostrea gigas; Mortalités; OsHV-1; Gènes viruax; Interaction hôte-virus; Transcriptome; Sensibilité; PCR en temps réel; Bivalve; Pacific oyster; Crassostrea ggias; Mortality; OsHV-1; Viral gene; Interaction host-virus; Transcriptome; Susceptibility; Real-time PCR.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00252/36297/34826.pdf
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Low pH reduced survival of the oyster Crassostrea gigas exposed to the Ostreid herpesvirus 1 by altering the metabolic response of the host ArchiMer
Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice.
Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary...
Tipo: Text Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf
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Impact of the oyster Crassostrea gigas on a microbial community in Atlantic coastal ponds near La Rochelle ArchiMer
Dupuy, Christine; Pastoureaud, Annie; Ryckaert, Mireille; Sauriau, Pierre-guy; Montanie, Hélène.
To assess the in situ impact of oysters Crassostrea gigas on planktonic protist and bacteria communities and the potential contribution of protozoa to their food resource intake, the abundance and the diversity of protists and bacteria were followed in 2 Atlantic coastal ponds, with and without oysters. The protist biomass in such ponds was high, with a maximum in spring of 982 mug C l(-1) and a minimum in winter of 179 pg C l(-1). Whatever the season, the presence of oysters (20 m(-2) corresponding to an average of 23 mg dry weight m(-2)) induced a significant decrease in >5 pm protist abundance. On the contrary, planktonic organisms <5 pm, such as Chlorophyta flagellates and bacteria, developed similarly in both ponds. It can be assumed that such...
Tipo: Text Palavras-chave: Trophic link; Bacteria; Protists; Microbial food web; Coastal pond; Food source; Oysters; Bivalve.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-822.pdf
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Derivation of toxicity equivalency factors for marine biotoxins associated with Bivalve Molluscs ArchiMer
Botana, Luis M.; Hess, Philipp; Munday, Rex; Nathalie, Arnich; Degrasse, Stacey L.; Feeley, Mark; Suzuki, Toshiyuki; Van Den Berg, Martin; Fattori, Vittorio; Gamarro, Esther Garrido; Tritscher, Angelika; Nakagawa, Rei; Karunasagar, Iddya.
Background Seafood toxins pose an important risk to human health, and maximum levels were imposed by regulatory authorities throughout the world. Several toxin groups are known, each one with many analogues of the major toxin. Regulatory limits are set to ensure that commercially available seafood is not contaminated with unsafe levels. Scope and Approach The mouse bioassay was used to measure the toxicity in seafood extracts to determine if a sample exceeded regulatory limits. The advantage of this approach was to provide an estimation of the total toxicity in the sample. As instrumental methods of analysis advance and serve as replacements to the mouse bioassay, the challenge is translating individual toxin concentrations into toxicity to determine...
Tipo: Text Palavras-chave: Marine toxins; Toxicity Equivalency Factors; FAO; WHO; Bivalve; Mollusc.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00358/46960/46874.pdf
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Growth Response of Arctica Islandica to North Atlantic Oceanographic Conditions Since 1850 ArchiMer
Poitevin, Pierre; Thébault, Julien; Siebert, Valentin; Donnet, Sébastien; Archambault, Philippe; Doré, Justine; Chauvaud, Laurent; Lazure, Pascal.
The Northwest Atlantic is a key region with an essential role in global climate regulation, redistributing heat and influencing the carbon cycle. However, little is known about its evolution before 1950, mainly because of the lack of long-term instrumental measurements. The hard parts of long-lived marine biota hold the potential to extend instrumentally derived observation by several decades or centuries and enhance our understanding of global climate processes. Here, we investigate the effects of local, regional, and large-scale climate variability on the marine bivalve, Arctica islandica (Linnaeus, 1767) from Saint-Pierre and Miquelon (SPM). This archipelago lies at the boundary zone between the cold Labrador Current in the north and the warm Gulf...
Tipo: Text Palavras-chave: Arctica islandica; Paleoecology; North Atlantic; Sub polar gyre; Labrador current; Bivalve; Sclerochronology; Climate change.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00509/62050/66198.pdf
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An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas ArchiMer
Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud.
This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,...
Tipo: Text Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf
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Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers ArchiMer
Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude.
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster...
Tipo: Text Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf
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Spatial distribution and nutritional requirements of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) in a Mediterranean Nanozostera noltii (Hornemann) meadow ArchiMer
Rossi, Francesca; Colao, Elodie; Jose Martinez, Maria; Klein, Judith C.; Carcaillet, Frederique; Callier, Myriam; De Wit, Rutger; Caro, Audrey.
Sulphur-oxidising endosymbiont-bearing bivalves often inhabit seagrass meadows, where they can control sulphide levels and variably contribute to carbon cycling, by feeding on endosymbiotic bacteria and/or on particulate organic matter from the water column. The patterns of variability in their feeding mode and their spatial distribution within the seagrass meadows are however poorly studied. Seagrass beds form naturally patchy habitats with seagrass-sand edges that may have variable effects on different organisms. The present study aims at understanding differences in feeding mode and abundance of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) as well as the physiological conditions of its endosymbiotic populations between edge and...
Tipo: Text Palavras-chave: Bivalve; Food web; Mediterranean; Seagrass; Stable isotopes; Symbiosis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00138/24911/26373.pdf
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Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane.
To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to...
Tipo: Text Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf
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Metabolism of the Pacific oyster, Crassostrea gigas , is influenced by salinity and modulates survival to the Ostreid herpesvirus OsHV-1 ArchiMer
Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice.
The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and...
Tipo: Text Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf
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Influence du mode de stockage sur la toxicité d'une eau et d'un sédiment à l'aide du bio-essai embryon de bivalve - Etude préliminaire ArchiMer
Damee, Nicolas.
La conservation des échantillons a souvent constitué un problème tant pour les chimistes que pour les biologistes. Si les chimistes semblent satisfaits de leurs techniques de conservation, les biologistes quant à eux sont indécis du fait que l'action toxique se modifie au cours du temps et selon la technique employée (réfrigération, congélation, lyophilisation, conservation en azote liquide à -196°C). Le présent rapport constitue une étude préliminaire qui, si elle est poursuivie, pourrait permettre de déterminer le mode de conservation le plus approprié pour chaque type d'échantillons.
Tipo: Text Palavras-chave: Conservation; Bio-essai; Pourcentage net d'anomalies (PNA); Sédiment; Bivalve.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00037/14828/12144.pdf
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French scallops: A new host for ostreid herpesvirus-1 ArchiMer
Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf
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